Bio-optical data

Bio-optical data will be processed to obtain inherent and apparent optical properties.

• Apparent Optical Properties;
  • Sub-surface remote sensing reflectance
    
  • Normalized water leaving radiance
    
  • Sub-surface Irradiance reflectance

• Inherent Optical Properties:
  • 1-meter binned profiles of absorption, scattering and total attenuation
    
  • 1-meter binned profiles of backscattering
• Biological and biogeochemical data
  • Inorganic nutrients are determined with a colorimetric method (Auto-analyzer);
  • Total N and P are determined with a colorimetric method (Auto-analyzer);;
  • Chlorophyll content is measured with spectrofluorimetric method;
  • Pigments are separated and quantyfied by HPLC analysis;
  • Particulate N and C is quantified with CHN elemental analyzer;
  • Primary production is obtained from $^{13}$C assimilation estimates;
  • Microzooplankton abundance is estimated from individual count;
  • Nanoflagellates concentration is obtained via epifluorescence;
  • Grazing and cannibalism of ciliates is estimated performing analyses on phytoplankton, nanoflagellates and microzooplankton on samples from different degrees of dilution;
  • Isotopic composition is measured by means of a mass spectrometer.